3D Bioprinting Applications in Tissue Engineering and Regenerative Medicine

Timeslot: Saturday, April 6, 2019 - 10:30am to 12:30pm
Track: Tissue Engineering and Regenerative Medicine
Room: Chelan 2

About

3D bioprinting is a fabrication technique used to mimic the anatomical complexity of native tissue, via a bottom-up approach, by depositing polymeric or cell-laden hydrogel based inks, in a layer-by-layer fashion. 3D bioprinting is a promising approachand to some of the most daunting obstacles facing the field of tissue engineering and regenerative medicine, including vascularization of tissue constructs, creation of complex architectures, and directing stem cell differentiation. The proposed session focuses on the recent advancements in 3D bioprinting technology in the development of complex, anatomical structures, motivating its use in a variety of biomedical applications such as regenerative medicine, tissue modeling, pharmacological assessment of therapeutics and modeling disease pathophysiology. Contributions regarding use of different bioprinting modality, along with recent development in advanced bioinks, are of interest to this session.

Abstracts

  • 10:30:00 AM 509. Printing Therapeutics in 3D Using Nanoengineered Bioink, A. Gaharwar*, C. Peak, J. Chen, M. Adlouni, K.A. Singh; Texas A&M University, College Station, TX, USA

  • 10:45:00 AM 510. Combinatorial Screening of 3D Printable Bioinks for Cartilage Repair, J. Galarraga*(1), S. Vega(2), M. Kwon(1), J. Burdick(1); (1)University of Pennsylvania, Philadelphia, PA, USA, (2)Rowan University, Glassboro, NJ, USA

  • 11:00:00 AM 511. Development of Open Source 3D Bioprinters For Low-Cost and High-Fidelity Biofabrication, J. Tashman*, S. Sohn, T. Hinton, D. Shiwarski, A. Lee, A. Hudson, A. Feinberg; Carnegie Mellon University, Pittsburgh, PA, USA

  • 11:15:00 AM 512. Engineering Cell Instructive DNA-Nanocomposites for Tissue Engineering, Bio-printing and Regenerative Medicine, A. Paul*; University of Kansas, Lawrence, KS, USA

  • 11:30:00 AM 513. 3D Bioprinting of Biomimetic Constructs with Spatially Controlled Microenvironment for Rotator Cuff Augmentation, B. Duan*, P. Streubel, M. Kuss, S. Wu; University of Nebraska Medical Center, Omaha, NE, USA

  • 11:45:00 AM 514. 3D Printing of Microstructured Collagen Scaffolds to Guide 3D Muscle Organization, A. Lee*, T. Hinton, A. Hudson, J. Bliley, A. Feinberg; Carnegie Mellon University, Pittsburgh, PA, USA

  • 12:00:00 PM 515. Fast Stereolithographic Printing of Large-scale Vascularized Tissue, R. Zhao*, N. Anandakrishnan, H. Ye, C. Zhou; State University of New York at Buffalo, Buffalo, NY, USA

  • 12:15:00 PM 516. Tunable Oxygen-Releasing, 3D-printed Scaffolds Improve in vivo Osteogenesis, A. Farris*, D. Lambrechts, N. Zhang, A. Rindone, E. Nyberg, A. O'Sullivan, S. Burris, K. Free, W. Grayson; Johns Hopkins University, Baltimore, MD, USA

Biomaterials for Therapeutic Drug Delivery 2

Timeslot: Saturday, April 6, 2019 - 10:30am to 12:30pm
Track: Therapeutic Delivery
Room: Skagit 4/5

About

Controlled release approaches have the potential to effectively treat a variety of medical conditions, while avoiding complications such as off-site toxicity and drug-resistance. Approaches can include localized, depot-based methods as well as targeted, systemic treatments. Controlled drug delivery can result from affinity interactions, bond cleavage, reservoir or diffusion-based control, and/or stimulus-responsive methodologies. This session will focus on the development of these drug delivery systems, which include nano and microparticles, hydrogels, scaffolds, and thin films, for applications including but not limited to regenerative medicine/tissue engineering, cancer, microbial infection, and autoimmune diseases.

Abstracts

  • 10:30:00 AM 465. Locally Anchoring Proteins to Target Tissues via Fusion to Galectin-3, S. Farhadi*, E. Bracho-Sanchez, M. Fettis, D. Seroski, S. Freeman, B. Keselowsky, G. Hudalla; University of Florida, Gainesville, FL, USA

  • 10:45:00 AM 466. Pulmonary Nanoparticle Delivery to Ameliorate Neuroinflammation of Experimental Autoimmune Encephalomyelitis, E. Saito*, S. Gurczynski, K. Kramer, C. Wilke, B. Moore, L. Shea; University of Michigan, Ann Arbor, MI, USA

  • 11:00:00 AM 467. Affinity Based Release of Protein Therapeutics to Treat Retinitis Pigmentosa, C. Teal*, V. Delplace, A. Ortin-Martinez, S. En-Leh Tsai, V. Wallace, M. Shoichet; University of Toronto, Toronto, ON, Canada

  • 11:15:00 AM 468. Liver Targeted Primaquine Drugamers for the Treatment of Malaria in High Risk Settings, S. Srinivasan*(1), J. Conner(2), R. Rochford(2), K. Montgomerry(1), C. LeGuyader(1), F. Su(1), P. Stayton(1), A. Convertine(3); (1)University of Washington, Seattle, WA, USA, (2)University of Colorado, Aurora, CO, USA, (3)Missouri University of Science an

  • 11:30:00 AM 469. Non-Invasive, Epicortical Delivery of Brain-Derived Neurotrophic Factor for Recovery After Stroke, E. Ho*, J. Obermeyer, A. Tuladhar, S. Payne, C. Morshead, M. Shoichet; University of Toronto, Toronto, ON, Canada

  • 11:45:00 AM 470. Compositional Control of pDNA/lPEI Nanoparticles Using Flash NanoComplexation to Improve in vivo Transfection Efficiency and Biocompatibility, Y. Hu*(1), H.-w. Liu(2), I. Minn(3), M. Pomper(3), H.-Q. Mao(1,2); (1)Johns Hopkins University School of Medicine, Baltimore, MD, USA, (2)Johns Hopkins University, Baltimore, MD, USA, (3)Johns Hopkins Medical Institutions, Baltimore, MD, USA

  • 12:00:00 PM 471. Tuning the Presentation and Density of Targeting Peptides on Polymeric Nanoparticles to Improve Bone Localization, M. Ackun-Farmmer*, Y. Yan, D. Fraser, D. Benoit; University of Rochester, Rochester, NY, USA

  • 12:15:00 PM 472. Towards a Nanoparticle-based Prophylactic for Maternal Autoantibody-related Autism, A. Bolandparvaz*, R. Harriman, N. Vapniarsky, K. Alvarez, Z. Zang, J. Van De Water, J. Lewis; University of California, Davis, Davis, CA, USA

Engineered Biomaterials for Neural Applications 2

Timeslot: Saturday, April 6, 2019 - 10:30am to 12:30pm
Track: Sensory Biomaterials and Tissues
Room: Skagit 2

About

Researchers are constantly developing and applying new biomaterials to challenging problems of the peripheral and central nervous systems. Engineered biomaterials are uniquely positioned for use in creating, testing, and regenerating neural tissue for better in vitro models of injury and disease, therapeutic treatments, understanding neural development, and mapping the brain. This session will focus on cutting edge research in neural biomaterials including fundamental material development through pre-clinical studies. These include big questions surrounding diseases and injuries spanning neurons, astrocytes, oligodendrocytes, microglia, and Schwann cells. Presentations will be highly interdisciplinary at the interfaces of biology, chemistry, materials science, engineering, and neuroscience. Target applications of these materials include neural injury, neurodegenerative diseases, stroke, diagnostics, brain-machine interfaces, and brain cancer.

Abstracts

  • 10:30:00 AM 493. Probing Extracellular Matrix Structural Changes in Organotypic Rat Brain Slices Using Multiple Particle Tracking, M. McKenna*(1), H. Pontes(1), E. Nance(1,2,3,4); (1)University of Washington Department of Chemical Engineering, Seattle, WA, USA, (2)University of Washington Department of Molecular Engineering & Sciences Institute, Seattle, WA, USA, (3)University of Was

  • 10:45:00 AM 494. Hyaluronic Acid-Based Hydrogels as a 3D Culture Platform for Neural Stem/Progenitor Cells, S. Seidlits*, C. Walthers, J. Liang, R. Bierman, A. Sohrabi, J. Karam; University of California Los Angeles, Los Angeles, CA, USA

  • 11:00:00 AM 495. Ile-Lys-Val-Ala-Val and Leu-Arg-Glu Peptide Signaling in Combination with Matrix Metalloprotease Cleavable Crosslinkers Alter Extracellular Matrix Degrading Enzyme Expression and Axon Extension in Encapsulated Human Induced Pluripotent Stem Cell Derived n, L. Smith Callahan*, T.H. Perera; University of Texas Health Science Center at Houston, Houston, TX, USA

  • 11:15:00 AM 496. Redesigning SDF-1 Delivery Strategies with in vitro, in vivo, and in silico Analyses, K. Hickey*, S. Grassi, M. Caplan, S. Stabenfeldt; Arizona State University, Tempe, AZ, USA

  • 11:30:00 AM 497. SilkBridge: A Novel Biomimetic and Biocompatible Silk-based Nerve Conduit, G. Bassani*(1), M. Biagiotti(1), L. Muratori(2,3), V. Vincoli(1), G. Ronchi(2,3), A. Alessandrino(1), S. Geuna(2,3), G. Freddi(1); (1)Silk Biomaterials Srl, Lomazzo, Italy, (2)University of Torino, Orbassano, Italy, (3)Neuroscience Institute Cavalieri Ott

  • 11:45:00 AM 498. Delivery of Neurotrophic Factors in a Silk-based Nerve Conduit for Peripheral Nerve Repair, C. de Carvalho(1,2,3), W. Chang(4), R. Reis(1,2,3), J. Oliveira*(1,2,3), J. Kohn(4); (1)3Bs Research group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Guimaraes, Portugal, (2)ICVS/3B’s–PT Government

  • 12:00:00 PM 499. Graphene-based Flexible Electronics Fabricated via Polymer Casting to Control Stem Cell Differentiation, M. Uz*, K. Jackson, M. Donta, J. Jung, D. Sakaguchi, J. Claussen, S. Mallapragada; Iowa State University, Ames, IA, USA

  • 12:15:00 PM 500. Fabrication of Nanofibrous Composite Meshes Incorporating Mg Metal particles for Nerve Repair Applications, N. Bhattarai*(1), U. Adhikari(1), X. An(2), S. Pixley(2), J. Sankar(1); (1)North Carolina A&T State University, Greensboro, NC, USA, (2)University of Cincinnati, Cincinnati, OH, USA

Engineered Microenvironments in Health and Disease

Timeslot: Saturday, April 6, 2019 - 10:30am to 12:30pm
Track: Engineered Microenvironments
Room: Chelan 4

About

Biomaterials have been invaluable tools for understanding how cells respond to their microenvironment in both health and disease. Here, we invite contributions that develop biomaterial platforms for cell culture or tissue engineering. Session topics include the following: understanding of the mechanisms that determine cellular and immune responses to disease/injury/biomaterials, determining how biophysical and biochemical cues alter cellular behavior in 3D, identifying differences between 2D and 3D microenvironments in mediating cellular phenotype or response to treatment, developing complex tissue microstructures/organioids, culturing multiple types of cells within complex microenvironments, driving or enriching specific populations, developing improved approaches for utilization of patient derived or difficult to culture cells, drug screening within engineered microenvironments, and engineering microenvironments for therapeutic purposes.

Abstracts

  • 10:30:00 AM 501. 3D Engineered Patient-Derived Xenograft Tumors to Recapitulate the Obese Colorectal Cancer Tumor Microenvironment, I. Hassani*, B. Anbiah, B. Ahmed, N. Habbit, M. Greene, E. Lipke; Auburn University, Auburn, AL, USA

  • 10:45:00 AM 502. Pulmonary Fibrotic Microtissue Array for Screening Anti-fibrosis Therapy, R. Zhao*, M. Asmani; State University of New York at Buffalo, Buffalo, NY, USA

  • 11:00:00 AM 503. Engineered Hydrogels to Induce Breast Cancer Dormancy and Reactivation, J. Slater*, S. Pradhan; University of Delaware, Newark, DE, USA

  • 11:15:00 AM 504. Microfluidic Gradient Generator for Drug Testing on a Colorectal Tumor-on-a-Chip Disease Model, M. Carvalho*(1,2,3), D. Barata(4), L. Teixeira(4), S. Giselbrecht(4), R. Reis(1,2,3), M. Oliveira(1,2,3), P. Habibovi?(4), R. Truckenmüller(4); (1)I3B’s Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Guimarães,

  • 11:30:00 AM 505. Cancer Associated Fibroblasts (CAFs) Confer Shear Resistance to Circulating Tumor Cells, A. Clinch*(1), N. Ortiz Otero(1,2), J. Marshall(2), M. King(1); (1)Vanderbilt University, Nashville, TN, USA, (2)Cornell University, Ithaca, NY, USA

  • 11:45:00 AM 506. Microfluidic Prostate Tumor-on-a-Chip Model for in vitro Recapitulation of the Tumor Microenvironment, N. Habbit*(1), B. Anbiah(1), L. Anderson(1), J. Suresh(1), I. Hassani(1), M. Eggert(2), S. Jasper(2), B. Prabhakarpandian(3), R. Arnold(2), E. Lipke(1); (1)Auburn University, Auburn University, AL, USA, (2)Auburn University Harrison School of Pharmacy, Au

  • 12:00:00 PM 507. Development of a 3D Hydrogel Model of Glioblastoma Invasion, L. Smith(1), L. Bahlmann*(1), L. Zhu(2), P. Dirks(3), M. Shoichet(1); (1)University of Toronto, Toronto, ON, Canada, (2)University of Calgary, Calgary, AB, Canada, (3)Hospital for Sick Children, Toronto, ON, Canada

  • 12:15:00 PM 508. Spatiotemporally Addressable Biomaterial Microenvironments for Modeling Pulmonary Fibrosis in vitro, C. Magin*(1,2), T. D'Ovidio(1), N. Darling(1); (1)University of Colorado, Anschutz Medical Campus, Aurora, CO, USA, (2)University of Colorado, Denver, Anschutz Medical Campus, Aurora, CO, USA

Functional Biomaterials to Control and Direct Cellular Function2

Timeslot: Saturday, April 6, 2019 - 10:30am to 12:30pm
Track: Functional Biomaterials and Surfaces
Room: Chelan 5

About

This session solicits abstracts that focus on the development functional biomaterials for tissue engineering applications. Specifically, studies that investigate biomaterial-directed cellular response in the absence of external factors (e.g., growth factors) will be highlighted. These studies include development of novel biomimetic approaches for the generation of tissue scaffolds with physicochemical properties (e.g., topography, composition, structure, mechanics) akin to the target tissue and assessment of the impact of these physicochemical cues on cell migration, proliferation, differentiation and de novo matrix production. Further, this session will also showcase in vivo studies that demonstrate the clinical translational capability of next generation functional biomaterials. Lastly, studies that focus on the investigation of biomaterial-based mechanisms that modulate cellular response are of interest.

Abstracts

  • 10:30:00 AM 485. Hyaluronic Acid-Based Hydrogels for Patient-Derived Xenograft Culture in a High Throughput Microfluidic Plate, D. Harrington*(1), L. Sablatura(2), K. Bircsak(3), P. Shepherd(4), N. Navone(4), P. Constantinou(4), A. Saleh(3); (1)The University of Texas Health Science Center, Houston, TX, USA, (2)Rice University, Houston, TX, USA, (3)MIMETAS, US, Gaithersburg, MD, U

  • 10:45:00 AM 486. Dissecting the Influences of Combinatorial Microenvironment on Hepatic Stellate Cell Behavior and Phenotype, I. Jain*(1), A. Brougham-Cook(1), D. Kukla(2), S. Khetani(2), G. Underhill(1); (1)University of Illinois at Urbana-Champaign, Urbana, IL, USA, (2)University of Illinois at Chicago, Chicago, IL, USA

  • 11:00:00 AM 487. High-throughput Mapping of Nanotopographies-Regulated Cell Behaviors by Large-scale Combinatorial Nanoarrays, L. Yang*, K.-B. Lee; Rutgers, the State University of New Jersey, Piscataway, NJ, USA

  • 11:15:00 AM 488. Microclot Array Elastometry for Integrated Measurement of Clot Biomechanics Under Fluid Shear, R. Zhao*, Z. Chen, J. Lu, C. Zhang, S. Neelamegham; State University of New York at Buffalo, Buffalo, NY, USA

  • 11:30:00 AM 489. Extracellular Matrix Enriched Spheroids for Enhanced Survival and Differentiation of Mesenchymal Stem Cells, T. Gonzalez-Fernandez*, A. Keown, A. Tenorio, K. Leach; University of California Davis, Davis, CA, USA

  • 11:45:00 AM 490. A Microfluidic Method for Creating Poly(ethylene glycol) Gradient Microgel Scaffolds to Modulate Human Mesenchymal Stem Cell Behavior, S. Xin*, J. Dai, A. Han, D. Alge; Texas A&M University, College Station, TX, USA

  • 12:00:00 PM 491. Photocrosslinked Bioglass Incorporated Methacrylated Collagen Hydrogels for Orthopedic Applications, N. Kajave*, T.-U. Nguyen, T. Schmitt, M. Fenn, V. Kishore; Florida Institute of Technology, Melbourne, FL, USA

  • 12:15:00 PM 492. Tendon Mimetic Magnetic Responsive Fibrous Scaffolds and Magneto-mechanical Stimulation Synergistically Promotes the Tenogenic Commitment of Human Adipose Derived Stem Cells, R. Domingues*(1,2,3), A. Tomás(1,2), A. Gonçalves(1,2), R. Reis(1,2,3), M. Gomes(1,2,3); (1)3B's Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, Guimarães, Portugal, (2)ICVS/3B’s–PT Government Associate Laboratory, Braga/Guimarães, Portugal, (3)The Discoveries Centre for Regenerative and Precision Medicine, Guimarães, Portugal

Immune Engineering SIG

Timeslot: Saturday, April 6, 2019 - 10:30am to 12:30pm
Track: Immunomodulatory Biomaterials
Room: Yakima 1

Abstracts

  • 10:30:00 AM 477. Human Pancreas Organ Slices as a Platform for Investigating the Immunopathological Processes of Type 1 Diabetes, E. Phelps*, C. Mathews; University of Florida, Gainesville, FL, USA

  • 10:45:00 AM 478. Apolipoproteins are a Mediator of Tissue-Specificity in the Foreign Body Response, K. Sadtler*(1,2), C. MacIsaac(1), F. Zepeda(1), J. Doloff(1,2), R. Langer(1,2), D. Anderson(1,2); (1)Massachusetts Institute of Technology, Cambridge, MA, USA, (2)Boston Children's Hospital, Harvard Medical School, Boston, MA, USA

  • 11:00:00 AM 479. Delivery of STING Agonist for Augmenting Adoptive T Cell Therapy, C. Park*(1,2), J. Moon(1,2,3); (1)Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA, (2)Biointerfaces Institute, University of Michigan, Ann Arbor, MI, USA, (3)Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, USA

  • 11:15:00 AM 480. Depleting Myeloid-derived Suppressor Cells with Janus Synthetic Nanoparticle Antibodies As A Cancer Immunotherapy, J. Liu(1,2), R. Toy*(1), P. Pradhan(1), K. Roy(1,2); (1)Georgia Institute of Technology, Atlanta, GA, USA, (2)Emory University, Atlanta, GA, USA

  • 11:30:00 AM 481. Self-Assembly of Immune Signals to Identify Tolerogenic Factors in a Multiple Sclerosis Model, R. Oakes*(1), L. Tostanoski(1), C. Jewell(1,2,3); (1)University of Maryland - College Park, College Park, MD, USA, (2)Robert E. Fischell Institute for Biomedical Devices, College Park, MD, USA, (3)Department of Veterans Affairs, Baltimore, MD, USA

  • 11:45:00 AM 482. Effects of Tunable Degradation on Humoral, Cellular, and Protective Immune Responses Generated by a Universal Flu Vaccine Formulated with Acetalated Dextran Microparticles, K. Ainslie*(1), N. Chen(2), M. Collier(3), M. Gallovic(4), J. Ting(1), E. Bachelder(1); (1)University of North Carolina, Chapel Hill, NC, USA, (2)Pfizer, Beijing, China, (3)Avanti Polar Lipids, Inc., Alabaster, AL, USA, (4)IMMvention Therapuetix, Durham,

  • 12:00:00 PM 483. Local Anchoring of Indoleamine 2, 3-Dioxygenase Ameliorates Inflammation, S. Freeman*(1), E. Bracho-Sanchez(1), F. Rocha(1), S. Farhadi(1), S. Wallet(2), G. Hudalla(1), B. Keselowsky(1); (1)University of Florida, Gainesville, FL, USA, (2)East Carolina University, Greenville, NC, USA

  • 12:15:00 PM 484. Polymeric Nanoparticles Improve Antibody Delivery to Tumors and Tumor Draining Lymph Nodes to Enhance Cancer Immunotherapy via Checkpoint Inhibition, D. Francis*, A. Schudel, N. Rohner, S. Thomas; Georgia Institute of Technology, Atlanta, GA, USA

Panel Discussion: Biomaterials in Industry: Past, Present, and Future

Timeslot: Saturday, April 6, 2019 - 10:30am to 12:30pm
Track: Career Catalysis
Room: Yakima 2

About

This panel discussion will feature speakers from the medtech industry, who will present their perspective on biomaterials that have had a commercial impact and on how biomaterials science will impact the medical products of the future. Audience participation in the discussion is encouraged. The target audience includes trainees interested in careers in industry and academic researchers with a translational focus. This session is jointly sponsored by SFB and the Americas Chapter of the Tissue Engineering and Regenerative Medicine International Society (TERMIS-AM).

Abstracts

  • 10:30 AM 473. Invited Speaker: TBD

  • 11:00 AM 474. Invited Speaker: TBD

  • 11:30 AM 475. Invited Speaker: TBD

  • 12:00 PM 476. Invited Speaker: TBD

Invited Speaker(s)

Tutorial: Best Practices for Immunohistochemistry - Foreign Body and Other Responses to Medical Devices

Timeslot: Saturday, April 6, 2019 - 10:30am to 12:30pm
Track: Thought Leader
Room: Skagit 3

About

A critical feature of biomaterials is how the body responds to the materials once exposed to the tissue. In order to improve the design and capabilities of biomaterials, a detailed understanding of the host response throughout the entire time of implant is needed. However, the common approaches, such as immunohistochemistry, to characterizing the host response can be experimentally tricky to apply, resulting in artifacts that can be highly misleading. Indeed, questions emerge regarding whether H&E is sufficient for full characterization. And if not, what alternative methodologies exist that can enhance our understanding of this critical response process. In this tutorial, experts in the field present an in-depth review on immunohistochemistry and foreign body tracking, sharing their experiences on techniques, pitfalls to avoid, and experimental instrumentation that can improve outcomes.

Abstracts

  • 10:30 AM 461. Invited Speaker: TBD

  • 11:00 AM 462. Invited Speaker: TBD

  • 11:30 AM 463. Invited Speaker: TBD

  • 12:00 PM 464. Invited Speaker: TBD

Invited Speaker(s)